Part:BBa_M36527:Experience

Unfortunately, we were unable to asses the performance of this part. Upon transformation, we found that our plates were consistently contaminated and only bacterial colonies grew. While the complete contamination made us unable to asses the functionality of the part, it may also indicate something about the part; perhaps this part does not make for very viable yeast colonies. If this is the case, this could likely be due to the fact that without LacI, the promoter works constitutively. Perhaps the promoter is so active without LacI to repress it (as it was after we attempted our initial transformation since it was still lacking the plasmid to express LacI) that the cells cannot properly grow up or multiply. If any group wanted to use this part, I would highly suggest incorporating the components necessary to also produce LacI (a promoter, kozak sequence, LacI gene, followed by a poly a tail) in the same plasmid. This would save a lot of time as a double transformation would not be necessary and may improve the potential for success of this part.

Applications of BBa_M36527

Originally, we had intended for this part (along with a LacI expressing plasmid) to be used as a lactose sensor. This could be a useful tool for lactose intolerant individuals as lactose levels in dairy products can vary. In order for them to easily identify the lactose level of various products and figure out what they can personally eat, yeast with this sensor could ideally be added to a small sample, resulting in the expression of some sort of reporter.

User Reviews

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